爆乳一区二区三区无码-蜜臀亚洲av无码精品国产午夜.-亚洲欧美一区二区三区日产-热99re久久精品这里都是精品

您好,歡迎進入上海力敏實業有限公司網站!
一鍵分享網站到:
您現在的位置:首頁 >> 產品中心 >> 生化試劑、抗體、血清 >> 抗體/蛋白 >> SC-160649santacruz PCGF6 (E-15) 抗體*

santacruz PCGF6 (E-15) 抗體*

  • 更新時間:  2023-07-24
  • 產品型號:  SC-160649
  • 簡單描述
  • santacruz PCGF6 (E-15) 抗體*
    Epitope: Internal (h)
    Applications: WB, IF, ELISA
    Species: m, r, h
    Isotype: goat IgG
詳細介紹

santacruz PCGF6 (E-15) 抗體*

BACKGROUND
Polycomb group (PcG) proteins form multiprotein complexes that regulate
expression patterns of developmental and cell proliferation genes. Several
members of the PcG contain ring finger domains and are identified as a subclass
of RING finger proteins. The RING-type zinc finger motif is present in a
number of viral and eukaryotic proteins and is made of a conserved cysteinerich
domain that is able to bind two zinc atoms. Proteins that contain the
RING-type zinc finger conserved domain are generally involved in the ubiquitination
pathway of protein degradation. PCGF6 (polycomb group ring finger
6), also known as MBLR or RNF134, is a 350 amino acid nuclear protein
that is ubiquitously expressed and contains one RING-type zinc finger. PCGF6
acts as a transcriptional repressor and regulates the level of histone
H3K4Me3 by activating SmcY histone demethylase. PCGF6 exists as two
alternatively spliced isoforms.
REFERENCES
1. Kanno, M., Hasegawa, M., Ishida, A., Isono, K. and Taniguchi, M. 1995.
Mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional
negative regulator with tumor suppressive activity. EMBO J. 14:
5672-5678.
2. Satijn, D.P., Gunster, M.J., van der Vlag, J., Hamer, K.M., Schul, W.,
Alkema, M.J., Saurin, A.J., Freemont, P.S., van Driel, R. and Otte, A.P.
1997. RING1 is associated with the polycomb group protein complex and
acts as a transcriptional repressor. Mol. Cell. Biol. 17: 4105-4113.
3. Joazeiro, C.A. and Weissman, A.M. 2000. RING finger proteins: mediators
of ubiquitin ligase activity. Cell 102: 549-552.
4. Akasaka, T., Takahashi, N., Suzuki, M., Koseki, H., Bodmer, R. and Koga, H.
2002. MBLR, a new RING finger protein resembling mammalian Polycomb
gene products, is regulated by cell cycle-dependent phosphorylation. Genes
Cells 7: 835-850.
5. Tuckfield, A., Clouston, D.R., Wilanowski, T.M., Zhao, L.L., Cunningham,
J.M. and Jane, S.M. 2002. Binding of the RING polycomb proteins to specific
target genes in complex with the grainyhead-like family of developmental
transcription factors. Mol. Cell. Biol. 22: 1936-1946.
6. Online Mendelian Inheritance in Man, OMIM™. 2003. Johns Hopkins
University, Baltimore, MD. MIM Number: 607816. World Wide Web URL:
http://www.ncbi.nlm.nih.gov/omim/
7. Moore, R. and Boyd, L. 2004. Analysis of RING finger genes required for
embryogenesis in C. elegans. Genesis 38: 1-12.
8. Yang, Y., Lorick, K.L., Jensen, J.P. and Weissman, A.M. 2005. Expression
and evaluation of RING finger proteins. Meth. Enzymol. 398: 103-112.
9. Lee, M.G., Norman, J., Shilatifard, A. and Shiekhattar, R. 2007. Physical and
functional association of a trimethyl H3K4 demethylase and Ring6a/MBLR,
a polycomb-like protein. Cell 128: 877-887.
STORAGE
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
CHROMOSOMAL LOCATION
Genetic locus: PCGF6 (human) mapping to 10q24.33; Pcgf6 (mouse) mapping
to 19 C3.
SOURCE
PCGF6 (E-15) is an affinity purified goat polyclonal antibody raised against a
peptide mapping within an internal region of PCGF6 of human origin.
PRODUCT
Each vial contains 200 μg IgG in 1.0 ml of PBS with < 0.1% sodium azide
and 0.1% gelatin.
Blocking peptide available for competition studies, sc-160649 P, (100 μg
peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).
APPLICATIONS
PCGF6 (E-15) is recommended for detection of PCGF6 of mouse, rat and
human origin by Western Blotting (starting dilution 1:200, dilution range
1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range
1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range
1:30-1:3000); non cross-reactive with PCGF1, PCGF3 or PCGF5.
Suitable for use as control antibody for PCGF6 siRNA (h): sc-90663, PCGF6
siRNA (m): sc-152109, PCGF6 shRNA Plasmid (h): sc-90663-SH, PCGF6
shRNA Plasmid (m): sc-152109-SH, PCGF6 shRNA (h) Lentiviral Particles:
sc-90663-V and PCGF6 shRNA (m) Lentiviral Particles: sc-152109-V.
Molecular Weight of PCGF6: 60 kDa.
RECOMMENDED SECONDARY REAGENTS
To ensure optimal results, the following support (secondary) reagents are
recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020
(dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey
anti-goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™
Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent:
sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluorescence:
use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100-
1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400)
with UltraCruz™ Mounting Medium: sc-24941.
RESEARCH USE
For research use only, not for use in diagnostic procedures.
PROTOCOLS
 

 

上海力敏實業有限公司代理santacruz產品,santacruz PCGF6 (E-15) 抗體* 咨詢和選購


留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
主站蜘蛛池模板: 久久亚洲私人国产精品va| 久久不见久久见免费视频7| 蜜臀av一区二区| 成 人 h在线观看| julia无码中文字幕一区| 狠狠色噜噜狠狠狠狠色综合久av| 亚洲国产日韩在线人成蜜芽| 少妇粗大进出白浆嘿嘿视频| 又大又硬又黄的免费视频| 亚洲 欧美 变态 另类 综合 | 国产精品无码专区在线播放| 久久国产免费观看精品3| 精品人妻无码区在线视频| 国产乡下三级全黄三级bd| 久久精品国产清自在天天线| 美女极度色诱视频国产| 少妇大叫太大太爽受不了| 大地资源在线观看中文第二页| 亚洲avav天堂av在线网毛片| 精品无码人妻一区二区三区品| 伊在人天堂亚洲香蕉精品区 | 抽搐一进一出gif日本| 内射爽无广熟女亚洲| 97久久超碰国产精品2021| 国内揄拍国内精品少妇| 欧美大肥婆bbbww| av成人午夜无码一区二区| 精品超清无码视频在线观看| 国产精品一区二区在线观看| 国产一区二区三区精品视频| 亚洲国产成人精品无码区花野真一| 插鸡网站在线播放免费观看| 青青草原亚洲| 校花高潮抽搐冒白浆视频| 亚洲性色av私人影院无码| 玩弄放荡人妇系列av在线网站| 亚洲国产欧美在线人成 | 国产成人精品无码一区二区老年人| 久久精品99久久香蕉国产| 久激情内射婷内射蜜桃| 野花社区视频www官网|